Cite this dataset as
Leitão, A.B., Ramesh, A., Day, J.P., Hanna, N., Devi, A., Hayes, M.P., Jiggins, F.M. (2022). Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps v2. NERC EDS Environmental Information Data Centre. (Dataset). https://doi.org/10.5285/06ea87f3-476d-40fd-acce-e6923e786d48
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This dataset is available under the terms of the Open Government Licence 
Changes in gene expression and encapsulation in Drosophila melanogaster caused by exposure to parasitoid wasps v2
This is the most recent version of this dataset (view other versions )
Data consists of gene expression estimates and encapsulation rates in wild caught Drosophila melanogaster larvae following exposure to different treatments. Treatments include injection with wasp homogenate, injection with oil and no injection. Also provided are functional enrichment categories for differentially expressed genes and library generation and read mapping metrics. The data were produced under the grant: NE/P00184X/1 Why does Drosophila vary in susceptibility to parasitoid wasps.
Publication date: 2022-03-08
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Formats
Comma-separated values (CSV)
txt
Spatial information
- Study area
-
- Spatial representation type
- Tabular (text)
- Spatial reference system
- OSGB 1936 / British National Grid
Temporal information
- Temporal extent
-
2017-10-01 to 2017-10-31
Provenance & quality
Drosophila melanogaster larvae obtained from a wild caught population were exposed injected with wasp homogenate, oil or no manipulation was made. Encapsulation rates were measured in each case in the fly larvae. For RNA-sequencing, hemocyte and fat body tissues were isolated from third instar larvae 24 hours post experimental manipulation. RNA was isolated for sequencing. Sequenced reads were trimmed, mapped to the dm6 genome, number of reads mapping to each gene was counted, differential expression tests were applied, and functional enrichment was done.
Correspondence/contact details
Authors
https://orcid.org/0000-0001-9004-1831
Day, J.P.
University of Cambridge
Hanna, N.
University of Cambridge
Devi, A.
University of Cambridge
Hayes, M.P.
University of Cambridge
Other contacts
- Rights holder
-
University of Cambridge
- Custodian
-
NERC EDS Environmental Information Data Centreinfo@eidc.ac.uk
- Publisher
-
NERC EDS Environmental Information Data Centreinfo@eidc.ac.uk
Additional metadata
- Topic categories
- biota
- INSPIRE theme
- Habitats and Biotopes
- Keywords
- differential gene expression , Drosophila melanogaster , encapsulation , Evolutionary genetics , fat body , genetics , haemocyte , insect genomics , Leptopilina boulardi , parasite , parasitoid wasp , RNA-sequencing
- Funding
- Natural Environment Research Council Award: NE/P00184X/1
- Last updated
- 27 February 2024 16:20